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dc.contributor.authorLíbalová, Helena
dc.contributor.authorRossner, Pavel
dc.contributor.authorVrbová, Kristýna
dc.contributor.authorBrzicová, Táňa
dc.contributor.authorSikorová, Jitka
dc.contributor.authorVojtíšek-Lom, Michal
dc.contributor.authorBeranek, Vít
dc.contributor.authorKléma, Jiří
dc.contributor.authorCiganek, Miroslav
dc.contributor.authorNeča, Jiří
dc.contributor.authorPěnčíková, Kateřina
dc.contributor.authorMachala, Miroslav
dc.contributor.authorTopinka, Jan
dc.date.accessioned2017-01-05T08:24:01Z
dc.date.available2017-01-05T08:24:01Z
dc.date.issued2016
dc.identifier.citationInternational Journal of Molecular Sciences. 2016, vol. 17, issue 11, art. no. 1833.cs
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10084/116566
dc.description.abstractThis study used toxicogenomics to identify the complex biological response of human lung BEAS-2B cells treated with organic components of particulate matter in the exhaust of a diesel engine. First, we characterized particles from standard diesel (B0), biodiesel (methylesters of rapeseed oil) in its neat form (B100) and 30% by volume blend with diesel fuel (B30), and neat hydrotreated vegetable oil (NEXBTL100). The concentration of polycyclic aromatic hydrocarbons (PAHs) and their derivatives in organic extracts was the lowest for NEXBTL100 and higher for biodiesel. We further analyzed global gene expression changes in BEAS-2B cells following 4 h and 24 h treatment with extracts. The concentrations of 50 mu g extract/mL induced a similar molecular response. The common processes induced after 4 h treatment included antioxidant defense, metabolism of xenobiotics and lipids, suppression of pro-apoptotic stimuli, or induction of plasminogen activating cascade; 24 h treatment affected fewer processes, particularly those involved in detoxification of xenobiotics, including PAHs. The majority of distinctively deregulated genes detected after both 4 h and 24 h treatment were induced by NEXBTL100; the deregulated genes included, e.g., those involved in antioxidant defense and cell cycle regulation and proliferation. B100 extract, with the highest PAH concentrations, additionally affected several cell cycle regulatory genes and p38 signaling.cs
dc.format.extent2125312 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofseriesInternational Journal of Molecular Sciencescs
dc.relation.urihttp://dx.doi.org/10.3390/ijms17111833cs
dc.rights© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectdieselcs
dc.subjectalternative fuelscs
dc.subjectdiesel exhaust particlescs
dc.subjectorganic extractscs
dc.subjectgene expression profilescs
dc.titleComparative analysis of toxic responses of organic extracts from diesel and selected alternative fuels engine emissions in human lung BEAS-2B cellscs
dc.typearticlecs
dc.identifier.doi10.3390/ijms17111833
dc.rights.accessopenAccess
dc.type.versionpublishedVersioncs
dc.type.statusPeer-reviewedcs
dc.description.sourceWeb of Sciencecs
dc.description.volume17cs
dc.description.issue11cs
dc.description.firstpageart. no. 1833cs
dc.identifier.wos000388809600067


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© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
Kromě případů, kde je uvedeno jinak, licence tohoto záznamu je © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).