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dc.contributor.authorTrajerová, Markéta
dc.contributor.authorKriegová, Eva
dc.contributor.authorMikulková, Zuzana
dc.contributor.authorSavara, Jakub
dc.contributor.authorKudělka, Miloš
dc.contributor.authorGallo, Jiří
dc.identifier.citationOsteoarthritis and Cartilage. 2022, vol. 30, issue 12, p. 1583-1592.cs
dc.description.abstractBackground: Knee osteoarthritis (KOA) is a highly heterogeneous disease encompassing a wide range of clinical phenotypes. Phenotypes based on immune cells and protein pattern in synovial fluid (SF) and their relationship to clinical trajectories have not been described. Objective: To assess phenotypes based on immune cells and protein pattern of SF in KOA. Design: SF-derived immune cells were investigated in 119 patients with KOA using flow cytometry. Immune-phenotypes (iPhen) were determined by multivariate patient similarity network analysis and related to clinical trajectory (3-6 months post-sampling) along with protein pattern and macrophage chemokine receptors. Results: Four iPhen were detected based on the distribution of T-lymphocytes, monocyte-macrophage lineage cells and activated CD8+ T-lymphocytes. The 'activated' phenotype (n = 17) had high T-lymphocytes but low monocyte-macrophage lineage cells and neutrophils, all highly activated, and showed improved symptoms in 70% patients. The 'lymphoid progressive' phenotype (n = 31) had high neutrophils, low lymphocytes and monocyte-macrophage lineage cells, low activation and was associated with lower pain levels. The 'myeloid progressive' phenotype (n = 35) had high NK and monocyte-macrophage lineage cells but low T-lymphocytes and activation. The 'aggressive' phenotype (n = 36) had high lymphocytes, macrophages, NK cells and neutrophils and high activation, and only 39% of patients improved during follow-up. Low CXCR4 and CCR7 expression on macrophages and high CXCL10 in SF were linked to improved clinical trajectory. Conclusion: We identified four immune-phenotypes that were associated with different clinical trajectories in KOA patients. How these phenotypes can be targeted therapeutically deserves further investigation.cs
dc.relation.ispartofseriesOsteoarthritis and Cartilagecs
dc.rights© 2022 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.cs
dc.subjectknee osteoarthritiscs
dc.subjectsynovial fluidcs
dc.subjectprotein patterncs
dc.subjectflow cytometrycs
dc.titleKnee osteoarthritis phenotypes based on synovial fluid immune cells correlate with clinical outcome trajectoriescs
dc.description.sourceWeb of Sciencecs

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