Elektrochemická měření v body fluid kapalinách s využitím potenciostatu

Abstract

The bachelor thesis aims to get students acquainted with methods of electrochemical detection and preparation of simulated body fluids. As the first step, a literature search was conducted on the topics “Electrochemical detection” and “Simulated body fluid solutions”. Subsequently, the chronoamperometry technique was chosen for its potential to detect dissolved glucose in simulated blood plasma – Hank’s Balanced Salt Solution (HBSS), whose ionic concentrations are similar to those found in human blood plasma. The detection was done with the help of flow analysis, where the carrier liquid is mixed with glucose from the simulated blood plasma. Glucose is then captured and detected using a biosensor, a flow cell, and the PalmSens 4 potentiostat. In the experimental part, the response to glucose concentrations in NaCl solution was measured first. Then, four HBSS samples with different glucose concentrations were measured and the different effects on the electrochemical response were evaluated, including the temperature of the glucose sample, the carrier fluid flow rate, and the applied voltage on the biosensor.