Stabilita hybridního nanokompozitního materiálu oxid zinečnatý/vermikulit/chlorhexidin

Abstract

This diploma thesis deals with preparation of a series of nanocomposite materials based on zinc oxide/vermiculite/chlorhexidine prepared by ultrasonic synthesis and their subsequent characterization. The aim of this work was to monitor the stability of the antibacterial hybrid nanocomposite in physiological saline. Theoretical part of the diploma thesis deals with the types of hybrid nanocomposite materials and their applications with a focus on medical utilization, and with the stability of metal oxide nanoparticles in an aqueous solution. In the experimental part of this work, nanocomposite materials based on zinc oxide/vermiculite/chlorhexidine have been prepared and characterized using the following methods: X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray fluorescence spectroscopy (XRFS), particle size distribution and Zeta potential analysis. The chlorhexidine-containing materials have been also analyzed for total carbon concentration (TOC). The release of zinc ions and chlorhexidine from the prepared materials into the Rigner’s solution has been investigated using the dialysis bag method. The amount of the released zinc ions has been determined by atomic absorption spectroscopy (AAS) and the amount of the released chlorhexidine has been determined by UV-VIS spectroscopy.