Elektrostatické zvlákňovaní a charakterizace nanovlákenných vrstev na bázi derivátů kyseliny hyaluronové
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Vysoká škola báňská – Technická univerzita Ostrava
Abstract
Cílem bakalářské práce bylo připravit nanovlákenné vrstvy na bázi derivátů kyseliny hyaluronové metodou elektrostatického zvlákňování. K přípravě byly použity deriváty lauroyl hyaluronan a furanyl hyaluronan. V průběhu procesu elektrostatického zvlákňování bylo do vrstev inkorporováno modelové barvivo chlorofenolová červeň. Morfologie nanovlákenných vrstev byla charakterizována pomocí skenovací elektronové mikroskopie. Byla určena gramáž vrstev, botnavost vrstev v roztoku fosfátového pufru a bylo sledováno zachování nanovlákenné struktury. Byla stanovena enkapsulační účinnost modelového barviva. Kinetika uvolňování modelového barviva z polymerní matrice byla studována pomocí vsádkové metody s dialyzační membránou a uvolňováním barviva do gelu. Zvolená nanovlákenná matrice neměla na kinetiku uvolňování zásadní vliv. Vrstvy obsahující kyselinu hyaluronovou a lauroyl hyaluronan si oproti vrstvám obsahujícím furanyl hyaluronan nezachovaly nanovlákennou strukturu v roztoku fosfátového pufru, zachovaly si svou mechanickou pevnost a množství uvolněného barviva bylo nižší.
The aim of this bachelor thesis was to prepare nanofiber layers based on hyaluronic acid derivatives by electrospinning. The derivatives lauroyl hyaluronan and furanyl hyaluronan were used. During the electrospinning, the model dye chlorophenol red was incorporated into the layers. The morphology of the nanofiber layers was characterized by scanning electron microscopy. The yield of the electrospinning process, the swelling of the layers in the phosphate buffer solution and the preservation of the nanofiber structure were determined. The encapsulation efficiency of the model dye was also determined. The release of the model dye from the polymer matrix was studied using a dialysis method. The release of the dye into the gel was also monitored. The selected nanofiber matrix did not have significant influence on the release kinetics. The layers containing hyaluronic acid and lauroyl hyaluronan did not maintain the nanofiber structure in the phosphate buffer solution, kept their mechanical strength and the amount of dye released was lower compared to the layers containing furanyl hyaluronan.
The aim of this bachelor thesis was to prepare nanofiber layers based on hyaluronic acid derivatives by electrospinning. The derivatives lauroyl hyaluronan and furanyl hyaluronan were used. During the electrospinning, the model dye chlorophenol red was incorporated into the layers. The morphology of the nanofiber layers was characterized by scanning electron microscopy. The yield of the electrospinning process, the swelling of the layers in the phosphate buffer solution and the preservation of the nanofiber structure were determined. The encapsulation efficiency of the model dye was also determined. The release of the model dye from the polymer matrix was studied using a dialysis method. The release of the dye into the gel was also monitored. The selected nanofiber matrix did not have significant influence on the release kinetics. The layers containing hyaluronic acid and lauroyl hyaluronan did not maintain the nanofiber structure in the phosphate buffer solution, kept their mechanical strength and the amount of dye released was lower compared to the layers containing furanyl hyaluronan.