Elektrostatické zvlákňovaní a charakterizace nanovlákenných vrstev na bázi derivátů kyseliny hyaluronové

Abstract

The aim of this bachelor thesis was to prepare nanofiber layers based on hyaluronic acid derivatives by electrospinning. The derivatives lauroyl hyaluronan and furanyl hyaluronan were used. During the electrospinning, the model dye chlorophenol red was incorporated into the layers. The morphology of the nanofiber layers was characterized by scanning electron microscopy. The yield of the electrospinning process, the swelling of the layers in the phosphate buffer solution and the preservation of the nanofiber structure were determined. The encapsulation efficiency of the model dye was also determined. The release of the model dye from the polymer matrix was studied using a dialysis method. The release of the dye into the gel was also monitored. The selected nanofiber matrix did not have significant influence on the release kinetics. The layers containing hyaluronic acid and lauroyl hyaluronan did not maintain the nanofiber structure in the phosphate buffer solution, kept their mechanical strength and the amount of dye released was lower compared to the layers containing furanyl hyaluronan.