Metody charakterizace chemického a fázového složení lidské plodové vody

Abstract

The thesis deals with experimental methods for body fluids analysis with a view to human amniotic fluids and their chemical characterization. Amniotic fluids of healthy fetuses and also amniotic fluids of fetuses with diagnosed congenital anomalies were analysed. Confocal Raman spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction analysis and scanning electron microscopy were utilized for the experimental characterization. Using Raman spectroscopy and scanning electron microscopy presence of several metals (or their compounds) in form of solid particles were detected. These metals are probably not the natural components of these bodyfluids. As a result of the comparison of chemical and phase analysis data of the amniotic fluids of healthy fetuses with the amniotic fluids of fetuses with congenital malformations, it can be stated that some differences were found. Metal-based solid particles detected were slightly more abundant in the samples with diagnosed malformations. Nonetheless, there is no convincing proof that there is a direct relationship between the presence of these particles and the origination of a malformation.