Vliv složení a postprocesních úprav nanovlákenných vrstev z derivátu kyseliny hyaluronové na profil uvolňování inkorporovaných látek

Abstract

The aim of this diploma thesis was to prepare self-supporting nanofibrous layers from a combination of modified hyaluronan (lauroyl hyaluronan, LAU) and polyvinylalcohol (PVA) by electrospinning (ES) and to compare release profile of the incorporated model substance in different forms. The effect of thermal cross-linking of PVA on the properties of the prepared layers was studied. The model dye Nile Red (NR) was incorporated into the nanofibrous layers in two ways (ES of blend solution and incorporation of NR into micellar aggregates). The prepared layers were characterized by scanning electron microscopy, confocal fluorescence microscopy, dynamic light scattering and differential scanning calorimetry. The encapsulation efficiency of NR and stability of the layers in distilled water were evaluated. The release of NR from polymer matrix into a medium simulating exudate (phosphate buffer with the addition of bovine serum albumin) was studied. The medium served as approximation of the environment for real application in wound healing. The obtained release profiles were compared, the effect of cross-linking and the presence of a secondary carrier on the release were discussed.