Vliv respirabilní frakce suspendovaných částic v ovzduší na funkce buněk imunitního systému člověka

Abstract

Immunomodulatory effects of a real airborne particulate sample were assessed using four laboratory immunoassays, allowing the evaluation of parameters of both the specific and nonspecific immune response and the allergy response. Assays were done in whole blood cultures from three healthy volunteers. The test results did not prove an influence of short-term effects of airborne particulate matter on immune cells´ functions of healthy persons. The presence of the aqueous suspension of particulate matter did not have an allergizing potential in the basophil degranulation assay, did not increase the phagocytic activity in the chemiluminescence assay and did not stimulate T-lymphocyte proliferation in the lymphocyte blast transformation assay. Likewise the results of the evaluation of cytokine production by a multiplex technology ALBIA show that the tested sample of airborne particulate matter does not belong to potent inductors of the immune system functions.