Příprava nanovlákenných vrstev s obsahem jílových minerálů pomocí elektrostatického zvlákňování.

Abstract

The aim of this diploma thesis was to prepare self-supporting homogenous nanofibrous layers with a presence of clay minerals vermiculite and kaolinite and these clay minerals modified by antibacterial agent chlorhexidine acetate (CH). Nanofibers were made of hydrophobic polymers, polyurethane and polycaprolactone. Polymer suspensions for electrospinning contained 2, 5 and 8 wt. % (according to the total weight of the suspension) of clay or CH/clay and were electrospun using 4SPIN LAB. Morphology of prepared nanofibrous layers was characterized using digital microscopy and scanning electron microscopy, EDX mapping and Raman spectroscopy was used to confirm the presence of clay minerals in the layer and their distribution. Fibre diameter decreased after adding the clay and was ranging from 600 nm to 5 μm. Antibacterial CH was found in the clay matrix as well as separately in the fibres (result of imperfect intercalation). Samples containing CH had good antibacterial activity against Staphylococcus aureus dependent upon CH release from the nanofiber mat.