Analýza metod uchycení enzymu na elektrodu pro měření glykémie

Abstract

The bachelor thesis deals with methods of enzyme immobilization, which are necessary for the measurement of blood glucose levels. Individual methods are analyzed and described in detail, similar to the functionality of the enzyme in the body. The method of immobilization was searched for in the research work, which was focused on the immobilization of the enzyme necessary for the measurement of glycemia, glucose oxidase. The chemical reactions influenced by the enzyme acting on the substrate to produce the product are also mentioned. The cross-linking method is selected from the above techniques. The aim of the work was to verify the immobilization of the enzyme by means of the produced electrode system, inserted into the correct measuring circuit.