Využití 3d tisku při studiu transportních jevů.

Abstract

This bachelor's thesis deals with 3D printing suitable for research membrane permeability, in our case for three different chemicals, which are nitrates, caffeine and bovine serum albumin and subsequent verifying of the functionality of the 3D cell and calculating the permeability of these chemical substances. The theoretical part deals with 3D printing from a general explanation to its usage nowadays. It also describes the theory of spectrophotometry, the theory of selected transport phenomena that can apply during the passage of substances membrane and primary properties of substances that were used for the measurements. The practical part describes the processes of making the design, printing, and completing the 3D cell, the construction of whole experiment, the preparation of the samples for membrane permeability measurements using UV-VIS spectrophotometer and subsequent permeability calculations. The conclusion summarizes the results of the permeability measurement, which amounts to 4,06·10-11 m2 s-1 for nitrate and 5,4·10-13 m2 s-1 for caffeine. Used BSA did not pass and its permeability is therefore 0.