Studium výskytu vybraných léčiv ve vodách z městské čistírny odpadních vod a optimalizace metod jejich detekce

Abstract

This dissertation focuses on the presence of non-steroidal anti-inflammatory drugs (NSAIDs) and their metabolites in wastewater, which are common contaminants and may pose a potential risk to the environment and human health. The dissertation focuses on the optimization of a selected analytical method for the detection of these substances, verification of its reliability through a validation process and its subsequent use in the annual monitoring of target analytes in wastewater samples from the Urban Wastewater Treatment Plant in Ostrava (WWTP). Optimised high-performance liquid chromatography coupled to a mass detector in a tandem arrangement was used for the determination of the monitored parameters, with the monitoring results demonstrating that the UWWTP effectively removes all monitored NSAIDs and their metabolites. The most frequently monitored influent concentrations of all target analytes ranged from 79-21 600 ng/l, these decreased after the UWWTP treatment process and at the outfall the most frequently observed values were in the range of 120-910 ng/l for diclofenac, 75-295 ng/l for ibuprofen and <160 ng/l for 4'-hydroxydiclofenac, carboxyibuprofen, naproxen and ketoprofen. The lowest mean removal efficiency was found for diclofenac, at 69%, and the highest for 4'-hydroxydiclofenac, ibuprofen and carboxyibuprofen, at 99%. In addition to the optimization of the analytical method for the determination of NSAIDs and their metabolites, the present dissertation also results in the first annual monitoring of these target analytes at a large municipal wastewater treatment plant, including a statistical evaluation of the data, which confirms their seasonal variation.