Mikro a nanometrické částice v lidských nádorových a hypertrofických tkáních

Abstract

The doctoral thesis deals with the detection of micro and nano-sized metallic particles in human hypertrophic tissues from carrier beams in patients diagnosed with chronic rhinitis, human lung cancer tissues and cytological specimens of sputum. The studied tissues and fluids were first characterized by the Raman microspectroscopy method, the selected samples were then analyzed by scanning electron microscopy, where the results were evaluated by statistical methods. In addition, road dust was collected depending on the traffic load (2 locations) in eight months, and analyzed as a potential source of particulate matter entering human respiratory system. These methods were able to detect the presence of solid particles / particle aggregates predominantly metal-based. Many of these were found in a size below 1 μm. Many of these particles / clusters of particles can be according to chemical composition classified by IARC as "probably carcinogenic to humans". The information and procedures thus obtained could further serve for medical practice. Characterized methods of particle detection in human tissues could thus prospectively be come a part of a special examination in some autoimmune diseases or in diseases that have been diagnosed as idiopathic, resulting from unknown causes