Publikační činnost Laboratoře výzkumu a managementu rizik / Publications of Laboratory for Risk Research and Management (023)

Permanent URI for this collectionhttp://hdl.handle.net/10084/64737

Kolekce obsahuje bibliografické záznamy publikační činnosti (článků) akademických pracovníků Laboratoře výzkumu a managementu rizik (023) v časopisech registrovaných ve Web of Science od roku 2003 do roku 2019. Útvar byl zrušen.
Do kolekce jsou zařazeny:
a) publikace, u nichž je v originálních dokumentech jako působiště autora (adresa) uvedena Vysoká škola báňská-Technická univerzita Ostrava (VŠB-TUO),
b) publikace, u nichž v originálních dokumentech není v adrese VŠB-TUO uvedena, ale autoři prokazatelně v době jejich zpracování a uveřejnění působili na VŠB-TUO.

Bibliografické záznamy byly původně vytvořeny v kolekci Publikační činnost akademických pracovníků VŠB-TUO, která sleduje publikování akademických pracovníků od roku 1990.

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Now showing 1 - 20 out of 27 results

Molecular responses in THP-1 macrophage-like cells exposed to diverse nanoparticles
(MDPI, 2019) Brzicová, Táňa; Javorková, Eliška; Vrbová, Kristýna; Zajícová, Alena; Holan, Vladimír; Pinkas, Dominik; Philimonenko, Vlada; Sikorová, Jitka; Kléma, Jiří; Topinka, Jan; Rössner Jr., Pavel
In the body, engineered nanoparticles (NPs) may be recognized and processed by immune cells, among which macrophages play a crucial role. We evaluated the effects of selected NPs [NM-100 (TiO2), NM-110 (ZnO), NM-200 (SiO2), and NM-300 K (Ag)] on THP-1 macrophage-like cells. The cells were exposed to subcytotoxic concentrations of NPs (1-25 mu g/mL) and the expression of immunologically relevant genes (VCAM1, TNFA, CXCL8, ICAM1, CD86, CD192, and IL1B) was analyzed by RT-qPCR. The expression of selected cytokines, growth factors and surface molecules was assessed by flow cytometry or ELISA. Generation of reactive oxygen species and induction of DNA breaks were also analyzed. Exposure to diverse NPs caused substantially different molecular responses. No significant effects were detected for NM-100 treatment. NM-200 induced production of IL-8, a potent attractor and activator of neutrophils, growth factors (VEGF and IGF-1) and superoxide. NM-110 triggered a proinflammatory response, characterized by the activation of transcription factor NF-kappa B, an enhanced production of proinflammatory cytokines (TNF-alpha) and chemokines (IL-8). Furthermore, the expression of cell adhesion molecules VCAM-1 and ICAM-1 and hepatocyte growth factor (HGF), as well as superoxide production and DNA breaks, were affected. NM-300 K enhanced IL-8 production and induced DNA breaks, however, it decreased the expression of chemokine receptor (CCR2) and CD86 molecule, indicating potential immunosuppressive activity. The toxicity of ZnO and Ag NPs was probably caused by their intracellular dissolution, as indicated by transmission electron microscopy imaging. The observed effects in macrophages might further influence both innate and adaptive immune responses by promoting neutrophil recruitment via IL-8 release and enhancing the adhesion and stimulation of T cells by VCAM-1 and ICAM-1 expression.
Inhalation of ZnO nanoparticles: Splice junction expression and alternative splicing in mice
(Oxford University Press, 2019) Rössner ml., Pavel; Vrbová, Kristýna; Strapáčová, Simona; Rössnerová, Andrea; Ambrož, Antonín; Brzicová, Táňa; Líbalová, Helena; Javorková, Eliška; Kulich, Pavel; Večeřa, Zbyněk; Mikuška, Pavel; Coufalík, Pavel; Křůmal, Kamil; Čapka, Lukáš; Dočekal, Bohumil; Moravec, Pavel; Šerý, Omar; Míšek, Ivan; Fictum, Petr; Fišer, Karel; Machala, Miroslav; Topinka, Jan
Despite the wide application of nanomaterials, toxicity studies of nanoparticles (NP) are often limited to in vitro cell models, and the biological impact of NP exposure in mammals has not been thoroughly investigated. Zinc oxide (ZnO) NPs are commonly used in various consumer products. To evaluate the effects of the inhalation of ZnO NP in mice, we studied splice junction expression in the lungs as a proxy to gene expression changes analysis. Female ICR mice were treated with 6.46 x 10(4) and 1.93 x 10(6) NP/cm(3) for 3 days and 3 months, respectively. An analysis of differential expression and alternative splicing events in 298 targets (splice junctions) of 68 genes involved in the processes relevant to the biological effects of ZnO NP was conducted using next-generation sequencing. Three days of exposure resulted in the upregulation of IL-6 and downregulation of BID, GSR, NF-kB2, PTGS2, SLC11A2, and TXNRD1 splice junction expression; 3 months of exposure increased the expression of splice junctions in ALDH3A1, APAF1, BID, CASP3, DHCR7, GCLC, GCLM, GSR, GSS, EHHADH, FAS, HMOX-1, IFN, NF-kB1, NQO-1, PTGS1, PTGS2, RAD51, RIPK2, SRXN1, TRAF6, and TXNRD1. Alternative splicing of TRAF6 and TXNRD1 was induced after 3 days of exposure to 1.93 x 10(6) NP/cm(3). In summary, we observed changes of splice junction expression in genes involved in oxidative stress, apoptosis, immune response, inflammation, and DNA repair, as well as the induction of alternative splicing in genes associated with oxidative stress and inflammation. Our data indicate the potential negative biological effects of ZnO NP inhalation.
Nano-TiO2 stability in medium and size as important factors of toxicity in macrophage-like cells
(Elsevier, 2019) Brzicová, Táňa; Sikorová, Jitka; Milcová, Alena; Vrbová, Kristýna; Kléma, Jiří; Pikal, Petr; Lubovská, Zuzana; Philimonenko, Vlada; Franco, F.; Topinka, Jan; Rössner Jr., Pavel
TiO2 along with nano-TiO2 are commonly found in consumer products. In vivo studies have observed an accumulation of nano-TiO2 in macrophages. However, characteristics of nano-TiO2 determining toxicity remain unclear. In our study, the cytotoxic effects of 14 diverse nano-TiO2 on THP-1 macrophage-like cells were measured by 3 cytotoxicity assays (MTS, WST-1 and LDH). Total averaged cytotoxicity was calculated using principal component analysis. Characteristics of all 14 nano-TiO2 included hydrodynamic diameter, zeta potential, shape, polydispersity index (PDI) and concentration; moreover, crystal form, specific surface area and crystallite size were measured for 10 nano-TiO2. The variables affecting cytotoxicity were chosen using LASSO (least absolute shrinkage and selection operator). Except for concentration, PDI in media measured within 1 h after preparation of the nanomaterial dispersion was selected as a variable affecting cytotoxicity: stable dispersion resulted in higher cytotoxic effects. Crystallite size has been shown to have nonlinear effects (particles of sizes between 20 and 60 nm were cytotoxic while smaller and larger ones were not) and thus it has been excluded from LASSO. The shape (particles/fibre) and crystal form did not affect the cytotoxicity. PDI and the nonlinear effect of size could be an explanation for the inconsistencies of the cytotoxicity of nano-TiO2 in various studies.
Cascading impact assessment in a critical infrastructure system
(Elsevier, 2018) Řehák, David; Šenovský, Pavel; Hromada, Martin; Loveček, Tomáš; Novotný, Petr
Research into disruptions to, or failures in, the Critical Infrastructure (further only CI), represents an important area of investigations into the phenomena in (a) Critical Infrastructure System (further only CIS). The results arising from the prediction of the intensity of problems - and the line(s) of their impacts spread-patterns, are an important part of any decision-making process carried out by the involved parties for the early and effective realization of Safety and Security Measures. Therefore, this article's aim is to assess cascading effects in a CI system. The first part of the article deals with the typology of impacts - the aspects that form their nature; and the ways these impacts spread in a CI structure. Furthermore, the current approaches to the assessment of such cascading impacts are also described. Based on these facts, the authors define the principles and framework for assessing cascading impacts in a CI system. The CIA Method (Cascading Impact Assessment - further only CIA), which serves for the quantification of the spread of cascading impacts in a CIS, is the most important part of this article. The essence of this method lies in its assessment of all lines of business occurring in the chosen area, as well as an assessment of their resilience and links; subsequent to this, a structural map of the risk of the spread of cascading impacts was created.
Verification of electronic device technology for measurement and evaluation of thermal exposure of fire fighters and members of rescue teams
(Nofer Institute of Occupational Medicine, 2018) Bernatíková, Šárka; Tomášková, Hana; Bužga, Marek; Jirák, Zdeněk; Novák, Petr; Babjak, Ján; Kot, Tomáš; Krys, Václav; Jánošík, Ladislav
Background: The work of members of rescue teams could be associated with very high physical and thermal loads. If not timely interrupted, any extreme labour-thermal load may lead to a failure of the body and fatal collapse. This risk may be significantly reduced by devices that monitor the response of the body during the intervention and inform rescuers about the need to interrupt the exposure when the critical value of the reference indicator is achieved. The aim of the study was to test the correlation between the data of the newly developed device for signaling the strain of rescuers and the indicators of physiological response of the body. Material and Methods: The tests were performed on 2 physically fit fire fighters dressed in a protective rescue suit and using insulating breathing apparatus, over a wide range of heat load under a model load on a bicycle ergometer in a climatic chamber. Results: The study provided a significant correlation between the body temperature measured in the ear canal and the temperature under the suit sensed by the tested device - the Safety Ambient Monitor (SAM) (R = 0.9007). The temperature under the suit also correlated with the temperature of the chest skin (R = 0.8928) and heart rate (R = 0.8613). Conclusions: A statistically significant correlation was proven between the temperature sensed by the SAM and the body temperature. The technical solution of sensing the temperature under the suit using the verified SAM technology does not affect or limit fire fighters in their work and minimizes the possibility of damage to the sensor and signaling failures.
Regional development and climate change adaptation: a study of the role of legitimacy
(De Gruyter, 2016) Thorstensen, Erik; Forsberg, Ellen-Marie; Underthun, Anders; Danihelka, Pavel; Řeháček, Jakub
This paper presents results from a study of Czech Local Action Groups (LAGs), focusing on gaining knowledge about their internally perceived legitimacy and their potential role in local adaptation to climate change. Former studies on the role of governance networks in climate change adaptation have suggested that these networks' legitimacy are crucial for their success. In this article we provide an analytical framework that can be used to address different aspects of local governance networks which are important for their legitimacy and the way they are apt as instruments for climate change adaptation actions. We also present a survey among LAG members that provide empirical data that we discuss in the article. The framework and the data are discussed with reference to existing contributions in the intersection of legitimacy, governance networks and climate change adaptation. A specific aim is to provide research based recommendations for further improving LAGs as an adaptation instrument. In addition, knowledge is generated that will be interesting for further studies of similar local governance initiatives in the climate change adaptation context.
Evaluation of combustion mechanisms using global uncertainty and sensitivity analyses: A case study for low-temperature dimethyl ether oxidation
(Wiley, 2014) Tomlin, Alison S.; Agbro, Edirin; Nevrlý, Václav; Dlabka, Jakub; Vašinek, Michal
A global uncertainty analysis is performed for three current mechanisms describing the low-temperature oxidation of dimethyl ether (Aramco Mech 1.3, Metcalfe et al., Int J Chem Kinet 2013, 45, 638–675; Zheng et al., Proc Combust Inst 2005, 30, 1101–1109; Liu et al., Combust Flame 2013, 160, 2654–2668) with application to simulations of species concentrations (CH2O, H2O2, CH3OCHO) corresponding to existing data from an atmospheric pressure flow reactor and high-pressure ignition delays. When incorporating uncertainties in reaction rates within a global sampling approach, the distributions of predicted targets can span several orders of magnitude. The experimental profiles, however, fall within the predictive uncertainty limits. A variance-based sensitivity analysis is then undertaken using high dimensional model representations. The main contributions to predictive uncertainties come from the CH3OCH2 + O2 system, with isomerization, propagation, chain-branching, secondary OH formation, and peroxy–peroxy reactions all playing a role. The response surface describing the relationship between sampled reaction rates and predicted outputs is complex in all cases. Higher order interactions between parameters contribute significantly to output variance, and no single reaction channel dominates for any of the conditions studied. Sensitivity scatter plots illustrate that many different parameter combinations could lead to good agreement with specific sets of experimental data. The Aramco scheme is then updated based on data from a recent study by Eskola et al. (J Phys Chem A, in press), which presents quite different temperature and pressure dependencies for the rates of CH3OCH2O2 [RIGHTWARDS ARROW] CH2OCH2O2H and CH2OCH2O2H [RIGHTWARDS ARROW] OH+2CH2O compared with currently used values and includes well skipping channels. The updates from Eskola worsen the agreement with experiments when used in isolation. However, if the rate of the CH2OCH2O2H + O2 channel is subsequently reduced, very good agreement can be achieved. Owing to the complex nature of the response surface, the tuning of this channel remains speculative. Further detailed studies of the temperature and pressure dependence of the CH3OCH2O2 + O2, CH2OCH2O2H + O2 system are recommended to reduce uncertainties within current dimethyl ether mechanisms for low-temperature conditions.
CFD modelling for atmospheric pollutants/aerosols studies within the complex terrains of urban areas and industrial sites
(Inderscience Publishers, 2014) Střižík, Michal; Zelinger, Zdeněk; Nevrlý, Václav; Kubát, Pavel; Berger, Pavel; Černý, Alexandr; Engst, Pavel; Bitala, Petr; Janečková, Radmila; Grigorová, Eva; Bestová, Iva; Čadil, Jan; Danihelka, Pavel; Kadeřábek, Petr; Kozubková, Milada; Drábková, Sylva; Hartman, David; Bojko, Marian; Zavila, Ondřej
Computational fluid dynamic (CFD) modelling of pollution dispersion and chemical conversion to aerosol particles in the atmospheric boundary layer (ABL) has been studied. The investigation focused on the numerical modelling above complex orographic terrains of urban areas and industrial sites including the dispersion of toxic substances in the air as a result of accidents. A finite-rate model of chemical reactions, including the turbulence chemistry for modelling the reaction between nitric acid and ammonia, has been applied. As supporting experiments, online monitoring of the spatial distribution of pollutants and aerosols has been performed above real complex areas. Minimal detectable concentrations 8 µg m−3 (SO2), 20 µg m−3 (NO2), 2 µg m−3 (O3) and minimal detectable absorptivity 5 × 10−7 cm−1 (aerosols) have been reached.
Radon, carbon dioxide and fault displacements in central Europe related to the Tōhoku earthquake
(Oxford University Press, 2014) Briestenský, Miloš; Thinová, Lenka; Praksová, Renáta; Stemberk, Josef; Rowberry, Matthew D.; Knejflová, Zuzana
Tectonic instability may be measured directly using extensometers installed across active faults or it may be indicated by anomalous natural gas concentrations in the vicinity of active faults. This paper presents the results of fault displacement monitoring at two sites in the Bohemian Massif and Western Carpathians. These data have been supplemented by radon monitoring in the Mladeč Caves and by carbon dioxide monitoring in the Zbrašov Aragonite Caves. A significant period of tectonic instability is indicated by changes in the fault displacement trends and by anomalous radon and carbon dioxide concentrations. This was recorded around the time of the catastrophic MW = 9.0 Tōhoku Earthquake, which hit eastern Japan on 11 March 2011. It is tentatively suggested that the Tōhoku Earthquake in the Pacific Ocean and the unusual geodynamic activity recorded in the Bohemian Massif and Western Carpathians both reflect contemporaneous global tectonic changes.
Spalování odpadní biomasy v malých zařízeních
(Česká společnost chemická, 2014) Martiník, Lubomír; Drastichová, Vendula; Horák, Jiří; Jankovská, Zuzana; Krpec, Kamil; Kubesa, Petr; Hopan, František; Kaličáková, Zdeňka
The analysis of the risks of the firedamp leakage at the Ostrava-Karvina mine district
(Technická univerzita Košice, 2012) Beňová, Eva; Koudelková, Jaroslava; Prokop, Pavel; Danihelka, Pavel
The article deals with the method of the safety risks analysis related to the methane leakage from the underground. The method uses the simplicity of the index method and the demands and the accuracy of the French MOSAR method as well. The MOSAR method is a system and systematic complex direction to work out the risk analysis. The analysis of the possible risks falls into fundamental ideas of the method namely the systematic sequence. The method sense is calculated the possible transfers of some danger. The index methods take advantage of the objective evaluation of the real danger related to the methane leakage from the underground. The main objective of the method is a reduction of the probability of the contingency beginning related to the methane leakage from underground and of the reduction of the impact of the contingency; it means the risk management.
Implementation and problematic aspects of regulation REACH
(Česká společnost chemická, 2013) Přichystalová, Radka; Sikorová, Lucie; Krejsová, Hana; Danihelka, Pavel
Nanoparticles emission during brown coal briquettes combustion in small output furnaces
(Kaprint, 2011) Danihelka, Pavel; Hase, Veronika; Mička, Vladimír; Kubesa, Petr; Krpec, Kamil; Horák, Jiří; Lach, Karel
Platinové kovy z automobilových katalyzátorů v životním prostředí
(Česká společnost chemická, 2011) Sikorová, Lucie; Ličbinský, Roman; Adamec, Vladimír
Statistical model of quality of radon measurements using electret ion chamber detectors
(Oxford University Press, 2011) Dubčáková, Renáta; Praks, Pavel; Moučka, Ladislav
This paper presents a statistical model for estimating probability, which states that a quality of radon measurements using the electret ion chamber system RM-1 will be accurate. The quality of the electret measurement was modelled as a ratio between the reference radon concentration and values measured under real conditions with varied levels of indoor radon concentration and microclimate indoor conditions. It was stated that the accuracy tolerance of measured values is 20 % of the given reference value. To estimate the uncertainty of the statistical model, the exact confidence limits for the estimated probabilities are computed. The statistical model was confirmed by an independent set of measurements. Moreover, the effect of absolute humidity on the quality estimation of electret detectors are also statistically analysed and discussed. The results of the statistical model confirm that the electret system is robust and suitable for estimation of radon concentration.
Time-resolved Fourier transform emission spectroscopy of CF3Br and CF3CFHCF3 in a pulsed electrical discharge
(Springer, 2011) Ferus, Martin; Civiš, Svatopluk; Kubelík, Petr; Nevrlý, Václav; Bitala, Petr; Grigorová, Eva; Střižík, Michal; Kubát, Pavel; Zelinger, Zdeněk
The environmentally important decomposition of halogenated species CF3Br and CF3CHFCF3 in helium discharge plasma was investigated by time-resolved high-resolution Fourier transform infrared emission spectroscopy. Contrary to classical pyrolysis, a deeper fragmentation of precursors up to atoms and lower molecular species was observed. Excited molecular products CF, CF2 and CF4 achieved the maximal concentration in the afterglow. The high concentration of all these species is in agreement with a kinetic model based on radical chemistry. The non-detectable concentration of CF3 can be connected to its high reactivity and the formation of more stable products, CF4 and CF2, by addition or release of a fluorine atom, respectively. Other products included HF, HBr, CO and cyano compounds that were produced by secondary reactions with traces of water vapor, atmospheric oxygen and nitrogen present in original industrial samples as impurities.
Eureqa: software review
(Springer, 2011) Dubčáková, Renáta
First analysis of the high resolution FTIR spectrum of the v(2) band of the FCO2 radical at 970.2 cm(-1)
(Taylor & Francis, 2010) Perrin, Agnes; Střižík, Michal; Beckers, H.; Willner, H.; Zelinger, Zdeněk; Pracna, Petr; Nevrlý, Václav; Grigorová, Eva
Možnosti využití přístupu indexování latentní sémantiky při předpovídání finančních krizí
(Vysoká škola ekonomická v Praze, 2009) Hájek, Petr; Střižík, Michal; Praks, Pavel; Kadeřábek, Petr
Allan variance for optimal signal averaging-monitoring by diode–laser and CO2 laser photo-acoustic spectroscopy
(Elsevier, 2009) Skřínský, Jan; Janečková, Radmila; Grigorová, Eva; Střižík, Michal; Kubát, Pavel; Herecová, Lenka; Nevrlý, Václav; Zelinger, Zdeněk; Civiš, Svatopluk
The concept of the Allan variance has been utilized to test the detection abilities of two powerful experimental methods for trace gas monitoring: IR diode–laser and CO2 laser photo-acoustic spectroscopy. The detection of stable molecules (OCS, allene, CH3OH), and unstable species (ozone, short-lived free radical CN and molecular ion ArD+) have been compared. An approach for studies of the influence of the reactivity on the optimal averaging time for the minimum detectable concentration is demonstrated.

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